دورية أكاديمية
Spectrophotometric and standard addition methods for quantitative determination of dopamine hydrochloride and levodopa in tablets and ampoules
| العنوان: | Spectrophotometric and standard addition methods for quantitative determination of dopamine hydrochloride and levodopa in tablets and ampoules |
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| المؤلفون: | Mohamed, Gehad, Nour-El-Dien, Faten, El-Nahas, Reham |
| المصدر: | Afinidad. Revista de química teórica y aplicada; Vol. 66, Núm. 541 (2009) Afinidad; Vol. 66, Núm. 541 (2009) |
| Publication Status: | published |
| بيانات النشر: | Universitat Ramon Llull, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Spectrophotometry, standard addition method, dopamine hydrochloride, levodopa, universal buffer, 4-aminoantipyrine, tablets, ampoules. |
| الوصف: | A Simple, accurate and sensitive spectrophotometric method for determining dopamine hydrochloride (DO·HCl) and levodopa (LD) in either pure forms or in their commercially available pharmaceutical formularions is reported. The main idea of the method is based on reacting a solutionof DO.HCl or LD at pH 12; using universal buffer, with4-aminoantipyrine (4-AAP) to form pink coloured couplingdye product with λmax = 475 (ε = 1.46x104 l·mol-1·cm-1) or454 nm (1.05x104 l·mol-1·cm-1) for DO·HCl and LD drugs,respectively. Before carrying out Beer’s law, different experimental conditions like time, temperature, sequence ofaddition and pH are optimized. The molar ratio method revealeda 1:1 [active ingredient]:[4-AAP] coupling product.The common excipients used as additives in pharmaceuticalsare examined in our proposed procedure as interferingmaterials. The calibration curves were developed by usingstandard DO·HCl and LD with percent recovery of 98.62– 102.9 and 97.40 – 101.5%, respectively. Beer’s law wasobeyed in the concentration range from 37.90 – 170.6 and49.30 – 221.8 mg/L of DO·HCl and LD, respectively. Thereproducibility and accuracy of the method was checkedby the values of SD = 0.04-0.22 and 0.05-0.15 and RSD= 0.06-0.16 and 0.02-0.10% for DO·HCl and LD, respectively.The results compare favourably with those of official and reported methods as indicated by the t- and F-test values, indicating the possibility of applying this spectrophotometricmethod in routine measurements. |
| نوع الوثيقة: | article |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2339-9686 0001-9704 |
| Relation: | https://www.raco.cat/index.php/afinidad/article/view/277273/365198 |
| URL الوصول: | https://www.raco.cat/index.php/afinidad/article/view/277273 |
| رقم الأكسشن: | edsrac.277273 |
| قاعدة البيانات: | RACO (Revistes Catalanes amb Accés Obert) |
| تدمد: | 23399686 00019704 |
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