| الوصف: |
This study aimed to investigate the effects of a biological nitrification inhibitor on nitrous oxide emission and rice yield quality in paddy soils and its effects on denitrifying the bacteria of nosZ, nirK, and nirS types. Two treatments were performed: (1) using a local conventional fertilizer as the control CK; (2) using the partial application of a conventional fertilizer + biological nitrification inhibitor as SW. N 2 O emission was measured using gas chromatography; qPCR amplification was performed using primers for the targeted functional genes, nosZ, nirS, and nirK, and denitrifying functional gene abundance and denitrifying microbial community structure were analyzed using fluorescence quantification and high–throughput sequencing, respectively. The results reveal that the biological nitrification inhibitor resulted in a 41.83% reduction in N 2 O, relative to the normal fertilizer treatment. Meanwhile, rice yield increased by 15.45% and related quality indexes were also improved. This can promote the reproduction of bacteria with the nosZ gene while inhibiting the growth of bacteria with nirS and nirK genes. The core bacteria, Nitrosospira , Rhodanobacter , Bradyrhizobium , Tardiphaga , Rhodopseudomonas , and Paracoccus, positively correlated with N 2 O emissions, while core bacteria Azospirillum , Burkholderia , and Mesorhizobium negatively correlated with N 2 O emissions. Therefore, the application of a biological nitrification inhibitor could be an effective measure to promote rice yield and quality, reduce N 2 O emissions, and affect key denitrifying bacteria. |
