Laboratory phase-contrast nanotomography of unstained Bombus terrestris compound eyes
| العنوان: | Laboratory phase-contrast nanotomography of unstained Bombus terrestris compound eyes |
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| المؤلفون: | Romell, Jenny, Jie, V. W., Miettinen, A., Baird, E., Hertz, Hans |
| المصدر: | Journal of Microscopy. 283(1):29-40 |
| مصطلحات موضوعية: | bumblebee, compound eyes, laboratory nano-CT, phase contrast, system comparison, glucose, glutaraldehyde, nanotube, osmium tetraoxide, paraformaldehyde, phosphotungstic acid, acceleration, Article, basement membrane, Bombus terrestris, comparative study, compound eye, controlled study, electric field, electron microscopy, histology, illumination, image processing, micro-computed tomography, nonhuman, phase contrast microscopy, photoreceptor cell, reconstruction algorithm, signal noise ratio, transmission electron microscopy, animal, bee, devices, laboratory, procedures, synchrotron, x-ray computed tomography, Animals, Bees, Laboratories, Microscopy, Phase-Contrast, Osmium Tetroxide, Synchrotrons, Tomography, X-Ray Computed, X-Ray Microtomography |
| الوصف: | Imaging the visual systems of bumblebees and other pollinating insects may increase understanding of their dependence on specific habitats and how they will be affected by climate change. Current high-resolution imaging methods are either limited to two dimensions (light- and electron microscopy) or have limited access (synchrotron radiation x-ray tomography). For x-ray imaging, heavy metal stains are often used to increase contrast. Here, we present micron-resolution imaging of compound eyes of buff-tailed bumblebees (Bombus terrestris) using a table-top x-ray nanotomography (nano-CT) system. By propagation-based phase-contrast imaging, the use of stains was avoided and the microanatomy could more accurately be reconstructed than in samples stained with phosphotungstic acid or osmium tetroxide. The findings in the nano-CT images of the compound eye were confirmed by comparisons with light- and transmission electron microscopy of the same sample and finally, comparisons to synchrotron radiation tomography as well as to a commercial micro-CT system were done. |
| وصف الملف: | |
| URL الوصول: | https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-309236 https://doi.org/10.1111/jmi.13005 |
| قاعدة البيانات: | SwePub |
| تدمد: | 00222720 13652818 |
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| DOI: | 10.1111/jmi.13005 |