Rapid test for lung maturity, based on spectroscopy of gastric aspirate, predicted respiratory distress syndrome with high sensitivity
| العنوان: | Rapid test for lung maturity, based on spectroscopy of gastric aspirate, predicted respiratory distress syndrome with high sensitivity |
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| المؤلفون: | Verder, Henrik, Heiring, Christian, Clark, Howard, Sweet, David, Jessen, Torben E., Ebbesen, Finn, Bjoerklund, Lars J., Andreasson, Bengt, Bender, Lars, Bertelsen, Aksel, Dahl, Marianne, Eschen, Christian, Fenger-Gron, Jesper, Hoffmann, Stine F., Hoeskuldsson, Agnar, Bruusgaard-Mouritsen, Maria, Lundberg, Fredrik, Postle, Anthony D., Schousboe, Peter, Schmidt, Peter, Stanchev, Hristo, Sorensen, Lars |
| المصدر: | Acta Paediatrica. 106(3):430-437 |
| مصطلحات موضوعية: | Gastric aspirate, Lung surfactant, Mid-infrared spectroscopy, Prematurity, Respiratory distress syndrome |
| الوصف: | Aim: Respiratory distress syndrome (RDS) is a major cause of mortality and morbidity in premature infants. By the time symptoms appear, it may already be too late to prevent a severe course, with bronchopulmonary dysplasia or mortality. We aimed to develop a rapid test of lung maturity for targeting surfactant supplementation. Methods: Concentrations of the most surface-active lung phospholipid dipalmitoylphosphatidylcholine and sphingomyelin in gastric aspirates from premature infants were measured by mass spectrometry and expressed as the lecithin/sphingomyelin ratio (L/S). The same aspirates were analysed with mid-infrared spectroscopy. Subsequently, L/S was measured in gastric aspirates and oropharyngeal secretions from another group of premature infants using spectroscopy and the results were compared with RDS development. The 10-minute analysis required 10 mu L of aspirate. Results: An L/S algorithm was developed based on 89 aspirates. Subsequently, gastric aspirates were sampled in 136 infants of 24-31 weeks of gestation and 61 (45%) developed RDS. The cut-off value of L/S was 2.2, sensitivity was 92%, and specificity was 73%. In 59 cases, the oropharyngeal secretions had less valid L/S than gastric aspirate results. Conclusion: Our rapid test for lung maturity, based on spectroscopy of gastric aspirate, predicted RDS with high sensitivity. |
| وصف الملف: | electronic |
| URL الوصول: | https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-136627 https://liu.diva-portal.org/smash/get/diva2:1089870/FULLTEXT01.pdf |
| قاعدة البيانات: | SwePub |
| تدمد: | 08035253 16512227 |
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| DOI: | 10.1111/apa.13683 |