Stallion spermatozoa surviving freezing and thawing experience membrane depolarization and increased intracellular Na+
| العنوان: | Stallion spermatozoa surviving freezing and thawing experience membrane depolarization and increased intracellular Na+ |
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| المؤلفون: | Ortega Ferrusola, C., Anel-Lopez, L., Ortiz-Rodriguez, J. M., Martin Munoz, P., Alvarez, M., de Paz, P., Masot, J., Redondo, E., Balao da Silva, C., Morrell, J. M., Rodriguez-Martinez, Heriberto, Tapia, J. A., Gil, M. C., Anel, L., Pena, F. J. |
| المصدر: | Andrology. 5(6):1174-1182 |
| مصطلحات موضوعية: | ATP, cryopreservation, flow cytometry, horse, Na+-K+, ATPase, sperm |
| الوصف: | In order to gain insight of the modifications that freezing and thawing cause to the surviving population of spermatozoa, changes in the potential of the plasma membrane (Em) and intracellular Na+ content of stallion spermatozoa were investigated using flow cytometry. Moreover, caspase 3 activity was also investigated and the functionality of the Na+-K+ ATPase pump was investigated before and after freezing and thawing. Cryopreservation caused a significant (pamp;lt;0.001) increase in the subpopulation of spermatozoa with depolarized sperm membranes, concomitantly with an increase (pamp;lt;0.05) in intracellular Na+. These changes occurred in relation to activation of caspase 3 (pamp;lt;0.001). Cryopreservation reduced the activity of the Na-K+ pump and inhibition of the Na+-K+ ATPase pump with ouabain-induced caspase 3 activation. It is concluded that inactivation of Na+-K+ ATPase occurs during cryopreservation, an inhibition that could play a role explaining the accelerated senescence of the surviving population of spermatozoa. |
| وصف الملف: | electronic |
| URL الوصول: | https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-143075 https://liu.diva-portal.org/smash/get/diva2:1159446/FULLTEXT01.pdf |
| قاعدة البيانات: | SwePub |
| تدمد: | 20472919 20472927 |
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| DOI: | 10.1111/andr.12419 |