NK Cell Function and Antibodies Mediating ADCC in HIV-1-Infected Viremic and Controller Patients
| العنوان: | NK Cell Function and Antibodies Mediating ADCC in HIV-1-Infected Viremic and Controller Patients |
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| المؤلفون: | Johansson, Susanne E, Rollman, Erik, Chung, Amy W, Center, Rob J, Hejdeman, Bo, Stratov, Ivan, Hinkula, Jorma, Wahren, Britta, Kaerre, Klas, Kent, Stephen J, Berg, Louise |
| المصدر: | Viral immunology. 24(5):359-368 |
| مصطلحات موضوعية: | MEDICINE, MEDICIN |
| الوصف: | Natural killer (NK) cells have been suggested to play a protective role in HIV disease progression. One potent effector mechanism of NK cells is antibody-dependent cellular cytotoxicity (ADCC) mediated by antiviral antibodies binding to the Fc gamma RIIIa receptor (CD16) on NK cells. We investigated NK cell-mediated ADCC function and the presence of ADCC antibodies in plasma from 20 HIV-1-infected patients and 10 healthy donors. The HIV-positive patients were divided into two groups: six who controlled viremia for at least 8 y without treatment (controllers), and 14 who were persistently viremic and not currently on treatment. Plasma from both patient groups induced NK cell IFN-gamma expression and degranulation in response to HIV-1 envelope (Env) gp140-protein-coated cells. Patient antibodies mediating ADCC were largely directed towards the Env V3 loop, as identified by a gp140 protein lacking the V3 loop. Interestingly, in two controllers ADCC-mediating antibodies were more broadly directed to other parts of Env. A high viral load in patients correlated with decreased ADCC-mediated cytolysis of gp140-protein-coated target cells. NK cells from both infected patients and healthy donors degranulated efficiently in the presence of antibody-coated HIV-1-infected Jurkat cells. In conclusion, the character of ADCC-mediating antibodies differed in some controllers compared to viremic patients. NK cell ADCC activity is not compromised in HIV-infected patients. |
| وصف الملف: | electronic |
| URL الوصول: | https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-72265 https://liu.diva-portal.org/smash/get/diva2:458802/FULLTEXT01.pdf |
| قاعدة البيانات: | SwePub |
| تدمد: | 08828245 15578976 |
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| DOI: | 10.1089/vim.2011.0025 |