A model to study complement involvement in experimental retinal degeneration
| العنوان: | A model to study complement involvement in experimental retinal degeneration |
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| المؤلفون: | Mohlin, Camilla, Sandholm, Kerstin, Kvanta, Anders, Ekdahl, Kristina N., Johansson, Kjell, 1957 |
| المصدر: | Upsala Journal of Medical Sciences. 123(1):28-42 |
| مصطلحات موضوعية: | AMD, RPE, complement system, ocular diseases, retina |
| الوصف: | BACKGROUND: The complement system (CS) plays a role in the pathogenesis of a number of ocular diseases, including diabetic retinopathy (DR), glaucoma, uveitis, and age-related macular degeneration (AMD). Given that many of the complex eye-related degenerative diseases have limited treatment opportunities, we aimed to mimic the in vivo retinal degenerative process by developing a relevant co-culture system.METHOD AND MATERIALS: The adult porcine retina was co-cultured with the spontaneously arising human retinal pigment epithelial cells-19 (ARPE-19).RESULTS: Inflammatory activity was found after culture and included migrating microglial cells, gliosis, cell death, and CS activation (demonstrated by a minor increase in the secreted anaphylotoxin C3a in co-culture). CS components, including C1q, C3, C4, soluble C5b-9, and the C5a receptor, were expressed in the retina and/or ARPE cells after culture. C1q, C3, and CS regulators such as C4 binding protein (C4BP), factor H (CFH), and factor I (CFI) were secreted after culture.DISCUSSION: Thus, our research indicates that this co-culturing system may be useful for investigations of the CS and its involvement in experimental neurodegenerative diseases. |
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| URL الوصول: | https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-65202 https://doi.org/10.1080/03009734.2018.1431744 |
| قاعدة البيانات: | SwePub |
PDF full text from Publisher | تدمد: | 03009734 20001967 |
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| DOI: | 10.1080/03009734.2018.1431744 |