Structural studies of a polysaccharide from Vibrio parahaemolyticus strain AN-16000
| العنوان: | Structural studies of a polysaccharide from Vibrio parahaemolyticus strain AN-16000 |
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| المؤلفون: | Fontana, Carolina, Zaccheus, Mona V., Weintraub, Andrej, Ansaruzzaman, Mohammad, Widmalm, Göran |
| المصدر: | Carbohydrate Research. 432:41-49 |
| مصطلحات موضوعية: | Vibrio parahemolyticus, NMR, Dephosphorylation, Glycerol-1-phosphate |
| الوصف: | The structure of a polysaccharide from Vibrio parahaemolyticus strain AN-16000 has been investigated. The sugar and absolute configuration analysis revealed D-Glc, D-GalN, D-QuiN and L-FucN as major components. The PS was subjected to dephosphorylation with aqueous 40% HF to obtain an oligosaccharide that was analyzed by H-1 and C-13 NMR spectroscopy. The HR-MS spectrum of the oligosaccharide revealed a pentasaccharide composed of two Glc residues, one QuiNAc and one GalNAc, one FucNAc, as well as a glycerol moiety. The structure of the PS was determined using H-1, C-13, N-15 and P-31 NMR spectroscopy; inter-residue correlations were identified by H-1, C-13-heteronuclear multiple-bond correlation, H-1, H-1-NOESY and H-1, P-31-hetero-TOCSY experiments. The PS backbone has the following teichoic acid-like structure: -> 3)-D-Gro-(1-P-6)-beta-D-Glcp-(1 -> 4)-alpha-L-FucpNAc-(1 -> 3)-beta-D-QuipNAc-(1 -> with a side-chain consisting of alpha-D-Glcp-(1 -> 6)-alpha-D-GalpNAc-(1 -> linked to the O3 position of the FucNAc residue. |
| وصف الملف: | electronic |
| URL الوصول: | https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-133271 https://su.diva-portal.org/smash/get/diva2:957987/FULLTEXT01.pdf |
| قاعدة البيانات: | SwePub |
| تدمد: | 00086215 1873426X |
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| DOI: | 10.1016/j.carres.2016.06.004 |