أعرض في EDS

The complement regulator CD46 is bactericidal to Helicobacter pylori and blocks urease activity

التفاصيل البيبلوغرافية
العنوان: The complement regulator CD46 is bactericidal to Helicobacter pylori and blocks urease activity
المؤلفون: Basmarke-Wehelie, Rahma, Sjölinder, Hong, Jurkowski, Wiktor, Elofsson, Arne, Arnqvist, Anna, Engstrand, Lars, Hagner, Matthias, Wallin, Elin, Guan, Na, Kuranasekera, Hasanthi, Aro, Helena, Jonsson, Ann-Beth
المصدر: Gastroenterology. 141(3):918-928
مصطلحات موضوعية: AhpC, Stomach Cancer, Bacterial Infection, mikrobiologi, Microbiology
الوصف: BACKGROUND & AIMS: CD46 is a C3b/C4b binding complement regulator and a receptor for several human pathogens. We examined the interaction between CD46 and Helicobacter pylori (a bacterium that colonizes the human gastric mucosa and causes gastritis), peptic ulcers, and cancer.METHODS: Using gastric epithelial cells, we analyzed a set of H pylori strains and mutants for their ability to interact with CD46 and/or influence CD46 expression. Bacterial interaction with full-length CD46 and small CD46 peptides was evaluated by flow cytometry, fluorescence microscopy, enzyme-linked immunosorbent assay, and bacterial survival analyses.RESULTS: H pylori infection caused shedding of CD46 into the extracellular environment. A soluble form of CD46 bound to H pylori and inhibited growth, in a dose- and time-dependent manner, by interacting with urease and alkyl hydroperoxide reductase, which are essential bacterial pathogenicity-associated factors. Binding of CD46 or CD46-derived synthetic peptides blocked the urease activity and ability of bacteria to survive in acidic environments. Oral administration of one CD46 peptide eradicated H pylori from infected mice.CONCLUSIONS: CD46 is an antimicrobial agent that can eradicate H pylori. CD46 peptides might be developed to treat H pylori infection.
وصف الملف: electronic
URL الوصول: https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-63669
https://su.diva-portal.org/smash/get/diva2:451664/FULLTEXT02.pdf
قاعدة البيانات: SwePub
الوصف
تدمد:00165085
15280012
DOI:10.1053/j.gastro.2011.05.009