Cellular responses to T-2 toxin and/or deoxynivalenol that induce cartilage damage are not specific to chondrocytes
| العنوان: | Cellular responses to T-2 toxin and/or deoxynivalenol that induce cartilage damage are not specific to chondrocytes |
|---|---|
| المؤلفون: | Lei, Yang, Guanghui, Zhao, Xi, Wang, Yingting, Wang, Xialu, Lin, Fangfang, Yu, Goldring, Mary, Xiong, Guo, Lammi, Mikko, 1961 |
| المصدر: | Scientific Reports. 7(1):2231 |
| مصطلحات موضوعية: | Kashin-Beck disease, T-2 toxin, deoxynivalenol, chondrocyte, cellforskning, cell research, Medical Cell Biology, medicinsk cellbiologi, Pathology, patologi, Toxicology, toxikologi |
| الوصف: | The relationship between T-2 toxin and deoxynivalenol (DON) and the risk of Kashin-Beck disease is still controversial since it is poorly known about their selectivity in cartilage damage. We aimed to compare the cytotoxicity of T-2 toxin and DON on cell lines representative of cell types encountered in vivo, including human chondrocytes (C28/I2), human hepatic epithelial cells (L-02) and human tubular epithelial cells (HK-2). In addition, we determined the distribution of T-2 toxin and DON in Sprague-Dawley (SD) rats after a single dose exposure. T-2 toxin or DON decreased proliferation in a time- and concentration-dependent manner and their combination showed a similar antagonistic effect in C28/I2, L-02 and HK-2 cells. Moreover, we observed cell cycle arrest and apoptosis, associated with increased oxidative stress and decline in mitochondrial membrane potential induced by T-2 toxin and/or DON. In vivo study showed that T-2 toxin and DON did not accumulate preferentially in the knee joint compared to liver and kidney after an acute exposure in SD rats. These results suggest that T-2 toxin and/or DON inhibit proliferation and induce apoptosis through a possible mechanism involving reactive oxygen species-mediated mitochondrial pathway that is not specific for chondrocytes in vitro or joint tissues in vivo. |
| وصف الملف: | electronic |
| URL الوصول: | https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-135354 https://doi.org/10.1038/s41598-017-02568-5 https://umu.diva-portal.org/smash/get/diva2:1098377/FULLTEXT01.pdf |
| قاعدة البيانات: | SwePub |
| تدمد: | 20452322 |
|---|---|
| DOI: | 10.1038/s41598-017-02568-5 |