Single cell transcriptomic analysis of spinal Dmrt3 neurons in zebrafish and mouse identifies distinct subtypes and reveal novel subpopulations within the dI6 domain
| العنوان: | Single cell transcriptomic analysis of spinal Dmrt3 neurons in zebrafish and mouse identifies distinct subtypes and reveal novel subpopulations within the dI6 domain |
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| المؤلفون: | Iglesias Gonzalez, Ana Belen, Jakobsson, Jon E. T., Vieillard, Jennifer, Lagerström, Malin C., Kullander, Klas, 1966, Boije, Henrik |
| المصدر: | Frontiers in Cellular Neuroscience. 15 |
| مصطلحات موضوعية: | spinal cord, locomotor network, dmrt3a, Wt1a, development, Neuroscience, Neurovetenskap |
| الوصف: | The spinal locomotor network is frequently used for studies into how neuronal circuitsare formed and how cellular activity shape behavioral patterns. A population of dI6interneurons, marked by the Doublesex and mab-3 related transcription factor 3(Dmrt3), has been shown to participate in the coordination of locomotion and gaitsin horses, mice and zebrafish. Analyses of Dmrt3 neurons based on morphology,functionality and the expression of transcription factors have identified differentsubtypes. Here we analyzed the transcriptomes of individual cells belonging to theDmrt3 lineage from zebrafish and mice to unravel the molecular code that underliestheir subfunctionalization. Indeed, clustering of Dmrt3 neurons based on their geneexpression verified known subtypes and revealed novel populations expressing uniquemarkers. Differences in birth order, differential expression of axon guidance genes,neurotransmitters, and their receptors, as well as genes affecting electrophysiologicalproperties, were identified as factors likely underlying diversity. In addition, thecomparison between fish and mice populations offers insights into the evolutionarydriven subspecialization concomitant with the emergence of limbed locomotion |
| وصف الملف: | electronic |
| URL الوصول: | https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-455734 https://doi.org/10.3389/fncel.2021.781197 https://uu.diva-portal.org/smash/get/diva2:1602504/FULLTEXT01.pdf |
| قاعدة البيانات: | SwePub |
| تدمد: | 16625102 |
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| DOI: | 10.3389/fncel.2021.781197 |