Regulatory B cells producing IL-10 are increased in human tumor draining lymph nodes
| العنوان: | Regulatory B cells producing IL-10 are increased in human tumor draining lymph nodes |
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| المؤلفون: | Piersiala, Krzysztof, Hjalmarsson, Eric, da Silva, Pedro Farrajota Neves, Lagebro, Vilma, Kolev, Aeneas, Starkhammar, Magnus, Elliot, Alexandra, Marklund, Linda, Munck-Wikland, Eva, Margolin, Gregori, Georen, Susanna Kumlien, Cardell, Lars-Olaf |
| المصدر: | International Journal of Cancer. 153(4):854-866 |
| مصطلحات موضوعية: | B regulatory cells, oral cancer, tumor-draining lymph nodes |
| الوصف: | The contribution of different immune cell subsets, especially T cells, in anti-tumor immune response is well established. In contrast to T cells, the anti-tumor contribution of B cells has been scarcely investigated. B-cells are often overlooked, even though they are important players in a fully integrated immune response and constitute a substantial fraction of tumor draining lymph nodes (TDLNs) known also as Sentinel Nodes. In this project, samples including TDLNs, non-TDLNs (nTDLNs) and metastatic lymph nodes from 21 patients with oral squamous cell carcinoma were analyzed by flow cytometry. TDLNs were characterized by a significantly higher proportion of B cells compared with nTDLNs (P = .0127). TDLNs-associated B cells contained high percentages of naive B cells, in contrary to nTDLNs which contained significantly higher percentages of memory B cells. Patients having metastases in TDLNs showed a significantly higher presence of immunosuppressive B regulatory cells compared with metastasis-free patients (P = .0008). Elevated levels of regulatory B cells in TDLNs were associated with the advancement of the disease. B cells in TDLNs were characterized by significantly higher expression of an immunosuppressive cytokine-IL-10 compared with nTDLNs (P = .0077). Our data indicate that B cells in human TDLNs differ from B cells in nTDLNs and exhibit more naive and immunosuppressive phenotypes. We identified a high accumulation of regulatory B cells within TDLNs which may be a potential obstacle in achieving response to novel cancer immunotherapies (ICIs) in head and neck cancer. |
| وصف الملف: | electronic |
| URL الوصول: | https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-512488 https://doi.org/10.1002/ijc.34555 https://uu.diva-portal.org/smash/get/diva2:1801020/FULLTEXT01.pdf |
| قاعدة البيانات: | SwePub |
| DOI: | 10.1002/ijc.34555 |
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