Intestinal damage in enterohemorrhagic Escherichia coli infection.
| العنوان: | Intestinal damage in enterohemorrhagic Escherichia coli infection. |
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| المؤلفون: | Békassy, Zivile, Calderon Toledo, Carla, Leoj, Gustav, Kristoffersson, Ann-Charlotte, Leopold, Shana R, Perez, Maria Thereza, Karpman, Diana |
| المصدر: | Pediatric Nephrology. oct:2059-2071 |
| مصطلحات موضوعية: | Medicin och hälsovetenskap, Klinisk medicin, Pediatrik, Medical and Health Sciences, Clinical Medicine, Pediatrics |
| الوصف: | Enterohemorrhagic Escherichia coli (EHEC) infection leads to marked intestinal injury. Sigmoid colon obtained from two children during EHEC infection exhibited abundant TUNEL-positive cells. To define which bacterial virulence factors contribute to intestinal injury the presence of Shiga toxin-2 (Stx2), intimin and the type III secretion system were correlated with symptoms and intestinal damage. C3H/HeN mice were inoculated with Stx2-producing (86-24) and non-producing (87-23) E. coli O157:H7 strains and 86-24 mutants lacking eae, encoding intimin (strain UMD619) or escN regulating the expression of type III secretion effectors (strain CVD451). Severe symptoms developed in mice inoculated with 86-24 and 87-23. Few mice inoculated with the mutant strains developed severe symptoms. Strain 86-24 exhibited higher fecal bacterial counts, followed by 87-23, whereas strains UMD619 and CVD451 showed minimal fecal counts. More TUNEL-positive cells were found in proximal and distal colons of mice inoculated with strain 86-24 compared with strains 87-23 and CVD451 (p |
| وصف الملف: | electronic |
| URL الوصول: | https://lup.lub.lu.se/record/1688642 https://portal.research.lu.se/files/5371376/1711811.pdf http://www.ncbi.nlm.nih.gov/pubmed/20809220?dopt=Abstract http://dx.doi.org/10.1007/s00467-010-1616-9 |
| قاعدة البيانات: | SwePub |
Find this article in full text from Springer Verlag. | تدمد: | 1432198X |
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| DOI: | 10.1007/s00467-010-1616-9 |